Residual effect of antiseptics and disinfectants used in health care environments against Enterococcus faecium
DOI:
https://doi.org/10.55204/trc.v4i1.e374Keywords:
Disinfectants, antiseptics, Enterococcus faecium, residual effectAbstract
Introduction: The residual effect of antiseptics and disinfectants in healthcare environments represents a critical and important aspect of the prevention of the spread of pathogenic bacteria such as Enterococcus faecium (E. faecium).
Objective: This study aimed to evaluate the residual effect of disinfectants and antiseptics used in hospital care settings against E. faecium.
Methodology: The most widely used antiseptics and disinfectants in Ecuador were used according to the Biosafety Manual of the Ministry of Public Health (MSP by its Spanish acronym) in different time intervals: 20 minutes, one, three, six, twelve, and twenty-four hours using the Kirby Bauer technique to observe the formation of inhibition halos of the bacteria against the chemical product.
Results: The results allowed to observe that ethanol 70%, sodium hypochlorite 0.5%, potassium monopersulfate 1%, glutaraldehyde 2%, and povidone-iodine 10% did not present a residual effect because they did not generate a halo of inhibition. However, E. faecium demonstrated sensitivity to 0.4% quaternary ammonium, followed by 2% chlorhexidine, in which the bacteria showed high sensitivity. On the other hand, hydrogen peroxide 10 Vol., E. faecium, initially showed sensitivity, but after 12 hours, it became non-existent.
Conclusion: In conclusion, E. faecium proved to be resistant to most of the hospital disinfectants tested, except chlorhexidine and hydrogen peroxide, suggesting a potential risk for antimicrobial resistance.
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Hirose R, Itoh Y, Ikegaya H, Miyazaki H, Watanabe N, Yoshida T, et al. Evaluation of the residual disinfection effects of commonly used skin disinfectants against viruses: An innovative contact transmission control method. Environ Sci Technol [Internet]. 2021;55(23):16044–55. Disponible en: http://dx.doi.org/10.1021/acs.est.1c05296
Saavedra SY, Bernal JF, Montilla-Escudero E, Torres G, Rodríguez MK, Hidalgo AM, et al. Vigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019. Rev Panam Salud Publica [Internet]. 2020 [citado el 29 de marzo de 2024];44:1. Disponible en: http://dx.doi.org/10.26633/rpsp.2020.104
Monsalve A NG, Moscoso Gama JM. Resistencia Bacteriana a Desinfectantes en áreas comunes de oficinas. Revista de la Asociación Colombiana de Ciencias Biológicas [Internet]. 2021;60–74. Disponible en: http://dx.doi.org/10.47499/revistaaccb.v1i33.225
Chacón-Jiménez L, Rojas-Jiménez K. Resistencia a desinfectantes y su relación con la resistencia a los antibióticos. Acta méd costarric [Internet]. 2020 [citado el 29 de marzo de 2024];62(1):7–12. Disponible en: https://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0001-60022020000100007
Rogers LA, Strong K, Cork SC, McAllister TA, Liljebjelke K, Zaheer R, et al. The role of whole genome sequencing in the surveillance of antimicrobial resistant Enterococcus spp.: A scoping review. Front Public Health [Internet]. 2021;9. Disponible en: http://dx.doi.org/10.3389/fpubh.2021.599285
Azabache JCB, Navarrete-Mejía PJ. En época de pandemia: eficacia de los desinfectantes de uso hospitalario en áreas críticas. Rev habanera cienc médicas [Internet]. 2023 [citado el 29 de marzo de 2024];22(2):4474. Disponible en: https://revhabanera.sld.cu/index.php/rhab/article/view/4474
Pidot SJ, Gao W, Buultjens AH, Monk IR, Guerillot R, Carter GP, et al. Increasing tolerance of hospital Enterococcus faecium to handwash alcohols. Sci Transl Med [Internet]. 2018;10(452). Disponible en: http://dx.doi.org/10.1126/scitranslmed.aar6115
Cobos Valdes D. Bioseguridad en el contexto actual. Rev Cuba Hig Epidemiol [Internet]. 2021 [citado el 30 de marzo de 2024];58. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1561-30032021000100015
Gold NA, Mirza TM, Avva U. Alcohol Sanitizer. 2024 [citado el 30 de marzo de 2024]; Disponible en: https://pubmed.ncbi.nlm.nih.gov/30020626/
Alotaibi SMI, Ayibiekea A, Pedersen AF, Jakobsen L, Pinholt M, Gumpert H, et al. Susceptibility of vancomycin-resistant and -sensitive Enterococcus faecium obtained from Danish hospitals to benzalkonium chloride, chlorhexidine and hydrogen peroxide biocides. J Med Microbiol [Internet]. 2017;66(12):1744–51. Disponible en: http://dx.doi.org/10.1099/jmm.0.000642
Aldunate MF, Cárdenas J. Uso de antisépticos y potencial riesgo de resistencia antimicrobiana [Internet]. Ispch.cl. 2021 [citado el 26 de abril de 2024]. Disponible en: https://www.ispch.cl/newsfarmacovigilancia/19/images/parte06.pdf
Wieland N, Boss J, Lettmann S, Fritz B, Schwaiger K, Bauer J, et al. Susceptibility to disinfectants in antimicrobial-resistant and -susceptible isolates of Escherichia coli, Enterococcus faecalis and Enterococcus faecium from poultry-ESBL/AmpC-phenotype of E. coli is not associated with resistance to a quaternary ammonium. J Appl Microbiol [Internet]. 2017;122(6):1508–17. Disponible en: http://dx.doi.org/10.1111/jam.13440
Suchomel M, Lenhardt A, Kampf G, Grisold A. Enterococcus hirae, Enterococcus faecium and Enterococcus faecalis show different sensitivities to typical biocidal agents used for disinfection. J Hosp Infect [Internet]. 2019;103(4):435–40. Disponible en: http://dx.doi.org/10.1016/j.jhin.2019.08.014
Duarte B, Pereira AP, Freitas AR, Coque TM, Hammerum AM, Hasman H, et al. 2CS-CHX T operon signature of chlorhexidine tolerance among Enterococcus faecium isolates. Appl Environ Microbiol [Internet]. 2019;85(23). Disponible en: http://dx.doi.org/10.1128/aem.01589-19
Eryılmaz M, Akın A, Arıkan Akan O. Investigación de la eficacia de algunos desinfectantes contra Staphylococcus aureus y Enterococcus spp. nosocomiales. aislados. 2019; Disponible en: https://europepmc.org/article/med/21935778
Cuy Calderón Wendy Daniela, Gallego García Angélica Valeria, Figueroa Garcia, Yulliet. Efecto de protocolos de desinfección en pruebas microbiológicas y en la resistencia mecánica de los conos de gutapercha [Internet]. Handle.net. 2019 [citado el 30 de marzo de 2024]. Disponible en: https://hdl.handle.net/20.500.12494/12906
Maimone S. Desinfectantes de Hospital: Monopersulfato de Postasio [Internet]. CODEINEP. 2018 [citado el 30 de marzo de 2024]. Disponible en: https://codeinep.org/desinfectantes-de-hospital-monopersulfato-de-potasio/
Llanga Ayol KE, Tapia Vallejo VE, Arteaga Sarmiento SD. Efecto residual de desinfectantes de uso hospitalario frente a Acinetobacter baumannii. AD [Internet]. 6 de febrero de 2024 [citado 24 de abril de 2024];7(1.1):58-2. Disponible en: https://cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/2884
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